4BE8
NEDD4 HECT A889F structure
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-12-10 |
| Detector | DECTRIS PILATUS 2M |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 100.545, 100.545, 96.450 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 44.580 - 2.996 |
| R-factor | 0.2482 |
| Rwork | 0.246 |
| R-free | 0.29500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2xbb |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.787 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.580 | 3.100 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.070 | 0.700 |
| Number of reflections | 11622 | |
| <I/σ(I)> | 28.4 | 3.9 |
| Completeness [%] | 99.3 | 99.1 |
| Redundancy | 13 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 9 | 1.15 M POTASSIUM SODIUM TARTRATE, 0.1 M TRIS-HCL, PH 9.0 |
