4BE5
V. cholera biofilm scaffolding protein RbmA
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13B1 |
| Synchrotron site | NSRRC |
| Beamline | BL13B1 |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2012-07-21 |
| Detector | ADSC CCD |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 118.888, 118.888, 104.781 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 24.720 - 2.460 |
| R-factor | 0.18543 |
| Rwork | 0.183 |
| R-free | 0.23434 |
| Structure solution method | MAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.405 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELX |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.500 |
| High resolution limit [Å] | 2.460 | 2.460 |
| Rmerge | 0.070 | 0.380 |
| Number of reflections | 27736 | |
| <I/σ(I)> | 45.4 | 9.74 |
| Completeness [%] | 99.9 | 99 |
| Redundancy | 9.8 | 9.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 0.1 M BIS/TRIS, PH 6.5, 1.5 M NACL |
