4BDU
Bax BH3-in-Groove dimer (GFP)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-05-25 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 64 |
| Unit cell lengths | 112.314, 112.314, 293.270 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.959 - 2.998 |
| R-factor | 0.2165 |
| Rwork | 0.215 |
| R-free | 0.24600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.390 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.900 | 3.100 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.173 | 1.731 |
| Number of reflections | 41413 | |
| <I/σ(I)> | 17.74 | 2.26 |
| Completeness [%] | 99.5 | 95.7 |
| Redundancy | 20.7 | 17.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 10% PEG3350, 20% MPD, 0.5% CHAPS, 0.1 M TRIS PH 8.0 |
