4BDL
Crystal structure of the GluK2 K531A LBD dimer in complex with glutamate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I02 |
Synchrotron site | Diamond |
Beamline | I02 |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | ADSC CCD |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 97.568, 106.611, 113.904 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 33.924 - 1.750 |
R-factor | 0.1709 |
Rwork | 0.169 |
R-free | 0.20160 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1xxr |
RMSD bond length | 0.006 |
RMSD bond angle | 1.028 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 33.920 | 1.780 |
High resolution limit [Å] | 1.750 | 1.750 |
Rmerge | 0.070 | 0.770 |
Number of reflections | 59953 | |
<I/σ(I)> | 15.5 | 2.2 |
Completeness [%] | 99.8 | 99.9 |
Redundancy | 4.5 | 4.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 19% PEG 4,000, 3% PROPAN-2-OL, 80MM NA ACETATE |