4BD6
Bax domain swapped dimer in complex with BaxBH3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-02-14 |
| Detector | ADSC CCD |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 103.715, 103.715, 40.555 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 51.858 - 2.494 |
| R-factor | 0.2482 |
| Rwork | 0.246 |
| R-free | 0.28530 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | BAX DOMAIN SWAPPED DIMER INDUCED BY OCTYLMALTOSIDE |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.480 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 51.800 | 2.850 |
| High resolution limit [Å] | 2.490 | 2.490 |
| Rmerge | 0.060 | 1.693 |
| Number of reflections | 8067 | |
| <I/σ(I)> | 26.72 | 1.45 |
| Completeness [%] | 98.9 | 94.1 |
| Redundancy | 12.2 | 11.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 1.4 M SODIUM CITRATE, 0.1 M SODIUM CACODYLATE PH 5.75 |
