4BD2
Bax domain swapped dimer in complex with BidBH3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-12-05 |
| Detector | ADXV |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 101.962, 101.962, 37.548 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.981 - 2.206 |
| R-factor | 0.179 |
| Rwork | 0.176 |
| R-free | 0.22700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | BAX DOMAIN SWAPPED DIMER INDUCED BY OCTYLMALTOSIDE |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.973 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 51.000 | 2.430 |
| High resolution limit [Å] | 2.210 | 2.210 |
| Rmerge | 0.220 | 1.500 |
| Number of reflections | 10428 | |
| <I/σ(I)> | 9.66 | 2.02 |
| Completeness [%] | 99.8 | 98.8 |
| Redundancy | 13.9 | 14.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 1.4 M SODIUM CITRATE, 0.1 M SODIUM CACODYLATE PH 5.75 |
