4BCZ
Monomeric Human Cu,Zn Superoxide dismutase, loops IV and VII deleted, apo form.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | MAX II BEAMLINE I911-2 |
Synchrotron site | MAX II |
Beamline | I911-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-03-15 |
Detector | MARRESEARCH 165MM |
Spacegroup name | P 65 |
Unit cell lengths | 74.430, 74.430, 59.270 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 30.000 - 1.930 |
R-factor | 0.1718 |
Rwork | 0.169 |
R-free | 0.21950 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2xjk |
RMSD bond length | 0.008 |
RMSD bond angle | 1.121 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.980 |
High resolution limit [Å] | 1.930 | 1.930 |
Rmerge | 0.060 | 0.650 |
Number of reflections | 14115 | |
<I/σ(I)> | 21.4 | 3.5 |
Completeness [%] | 99.8 | 99.9 |
Redundancy | 6.7 | 6.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 293 | 30% PEG 1500, 0.1M MIB BUFFER, PH 4.0, 20 C |