4BCY
Monomeric Human Cu,Zn Superoxide dismutase, mutation H43F
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I911-2 |
| Synchrotron site | MAX II |
| Beamline | I911-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-02-15 |
| Detector | MARRESEARCH 165MM |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 34.870, 47.910, 81.180 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.193 - 1.272 |
| R-factor | 0.1918 |
| Rwork | 0.191 |
| R-free | 0.20340 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2xjk |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.096 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.300 |
| High resolution limit [Å] | 1.270 | 1.270 |
| Rmerge | 0.050 | 0.790 |
| Number of reflections | 36511 | |
| <I/σ(I)> | 15.5 | 2.3 |
| Completeness [%] | 99.9 | 99.8 |
| Redundancy | 5.8 | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 293 | 25% PEG 400, 0.1M NA ACETATE PH 4.6, 0.1 M CDCL2, 20 C |
