4BCU
Satellite Tobacco Necrosis Virus (STNV) virus like particle in complex with the B3 aptamer
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I02 |
Synchrotron site | Diamond |
Beamline | I02 |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | ADSC CCD |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 315.020, 300.550, 183.510 |
Unit cell angles | 90.00, 94.37, 90.00 |
Refinement procedure
Resolution | 217.150 - 2.290 |
R-factor | 0.15729 |
Rwork | 0.155 |
R-free | 0.19821 |
Structure solution method | OTHER |
Starting model (for MR) | NONE |
RMSD bond length | 0.023 |
RMSD bond angle | 1.756 |
Data reduction software | xia2 |
Data scaling software | xia2 |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 217.000 | 2.350 |
High resolution limit [Å] | 2.290 | 2.290 |
Rmerge | 0.080 | 0.390 |
Number of reflections | 756410 | |
<I/σ(I)> | 9.3 | 3.3 |
Completeness [%] | 99.6 | 99.2 |
Redundancy | 5.5 | 3.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6.5 | pH 6.5 |