4BCT
Crystal structure of kiwi-fruit allergen Act d 2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ELETTRA BEAMLINE 5.2R |
Synchrotron site | ELETTRA |
Beamline | 5.2R |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | MARRESEARCH |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 71.541, 48.312, 50.580 |
Unit cell angles | 90.00, 100.72, 90.00 |
Refinement procedure
Resolution | 50.000 - 0.980 |
R-factor | 0.11406 |
Rwork | 0.113 |
R-free | 0.13125 |
Structure solution method | OTHER |
Starting model (for MR) | NONE |
RMSD bond length | 0.025 |
RMSD bond angle | 2.394 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 0.980 |
Rmerge | 0.040 |
Number of reflections | 97776 |
<I/σ(I)> | 29.7 |
Completeness [%] | 99.0 |
Redundancy | 4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 22-30% PEG 6000, PH 6.8-7.5 |