4BCE
crystal structure of Ttb-gly N282T mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-11-30 |
| Detector | ADSC QUANTUM 4 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 143.167, 77.665, 122.015 |
| Unit cell angles | 90.00, 100.08, 90.00 |
Refinement procedure
| Resolution | 39.970 - 2.000 |
| R-factor | 0.15725 |
| Rwork | 0.156 |
| R-free | 0.19015 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ug6 |
| RMSD bond length | 0.026 |
| RMSD bond angle | 1.993 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.160 | 2.060 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.070 | 0.460 |
| Number of reflections | 86355 | |
| <I/σ(I)> | 11.7 | 2.6 |
| Completeness [%] | 97.5 | 99 |
| Redundancy | 3.1 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | SODIUM CITRATE 0.4M, SODIUM CACODYLATE 0.1M, PH 6.5 |
