4BC3
Crystal structure of human D-xylulokinase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | LNLS BEAMLINE W01B-MX2 |
| Synchrotron site | LNLS |
| Beamline | W01B-MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-08-13 |
| Detector | MARRESEARCH |
| Spacegroup name | P 32 |
| Unit cell lengths | 99.790, 99.790, 157.540 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 58.220 - 1.680 |
| R-factor | 0.1598 |
| Rwork | 0.159 |
| R-free | 0.18360 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | HUMAN D-XYLULOSE DETERMINED BY TWO-ENERGY SELENIUM MAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.040 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 58.250 | 1.710 |
| High resolution limit [Å] | 1.680 | 1.680 |
| Rmerge | 0.170 | |
| Number of reflections | 200085 | |
| <I/σ(I)> | 9.4 | 0.6 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 8.5 | 8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 200 MM MES/KOH, PH 5.9, 13% PEG 6000 |
