4BBY
MAMMALIAN ALKYLDIHYDROXYACETONEPHOSPHATE SYNTHASE: WILD-TYPE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 1 |
| Unit cell lengths | 61.295, 99.170, 107.835 |
| Unit cell angles | 90.43, 92.18, 94.92 |
Refinement procedure
| Resolution | 19.980 - 1.900 |
| R-factor | 0.19179 |
| Rwork | 0.191 |
| R-free | 0.23978 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2uuu |
| RMSD bond length | 0.023 |
| RMSD bond angle | 2.233 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0027) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.150 | 0.360 |
| Number of reflections | 184434 | |
| <I/σ(I)> | 7.4 | 2.3 |
| Completeness [%] | 91.8 | 78.4 |
| Redundancy | 2.7 | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | pH 7.5 |
