4BBS

Structure of an initially transcribing RNA polymerase II-TFIIB complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Detector	DECTRIS PILATUS 6M
Spacegroup name	C 2 2 21
Unit cell lengths	222.170, 386.010, 254.470
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.273 - 3.600
R-factor	0.1855
Rwork	0.185
R-free	0.22500
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.120
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	3.600
Rmerge	0.230
Number of reflections	126022
<I/σ(I)>	9.5
Completeness [%]	99.9
Redundancy	7.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1