4BBP
X-ray structure of zinc bound ZnuA in complex with RDS51
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-03-18 |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | P 63 |
| Unit cell lengths | 143.197, 143.197, 30.833 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 124.010 - 2.150 |
| R-factor | 0.19899 |
| Rwork | 0.197 |
| R-free | 0.23187 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2xy4 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.192 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0070) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.230 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Rmerge | 0.100 | 0.440 |
| Number of reflections | 19952 | |
| <I/σ(I)> | 29.7 | 5.06 |
| Completeness [%] | 99.1 | 97.2 |
| Redundancy | 13.5 | 12 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | AMMONIUM SULPHATE 2.6M, PEG 400 1.5%, HEPES 1M, PH 7.5 |
