4BB7
Crystal structure of the yeast Rsc2 BAH domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 199 |
| Detector technology | CCD |
| Collection date | 2009-04-10 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 64.090, 64.070, 136.840 |
| Unit cell angles | 90.00, 95.47, 90.00 |
Refinement procedure
| Resolution | 44.493 - 2.400 |
| R-factor | 0.2203 |
| Rwork | 0.217 |
| R-free | 0.28560 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1w4s |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.772 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.000 | 2.530 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.140 | 0.420 |
| Number of reflections | 42935 | |
| <I/σ(I)> | 5.8 | 2.7 |
| Completeness [%] | 98.7 | 99 |
| Redundancy | 3.5 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 100 MM HEPES.NAOH PH 7.5, 0.2 M (NH4)2SO4, 20-30% W/V PEG 3350 |
