4BAX
Crystal structure of glutamine synthetase from Streptomyces coelicolor
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | MARMOSAIC 225 mm CCD |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 97.120, 113.000, 194.440 |
Unit cell angles | 90.00, 98.55, 90.00 |
Refinement procedure
Resolution | 24.996 - 2.550 |
R-factor | 0.2076 |
Rwork | 0.206 |
R-free | 0.23580 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.010 |
RMSD bond angle | 1.321 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 25.000 |
High resolution limit [Å] | 2.550 |
Rmerge | 0.090 |
Number of reflections | 134887 |
<I/σ(I)> | 12 |
Completeness [%] | 99.6 |
Redundancy | 3.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 |