4BAS
Structure of the Arl6 BBS3 Small GTPase from Trypanosoma brucei with bound nucleotide analogue GppNp
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-08-04 |
| Detector | ADSC CCD |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 70.920, 70.920, 80.928 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 61.420 - 2.000 |
| R-factor | 0.18658 |
| Rwork | 0.183 |
| R-free | 0.24783 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2h57 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.455 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MrBUMP |
| Refinement software | REFMAC (5.7.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.920 | 2.110 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.120 | 0.840 |
| Number of reflections | 15861 | |
| <I/σ(I)> | 15 | 3 |
| Completeness [%] | 96.7 | 100 |
| Redundancy | 10.6 | 11 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.2 M CSCL, 2.2 M (NH4)2SO4 |
