4BAF
Hen egg-white lysozyme structure in complex with the europium tris- hydroxyethyltriazoledipicolinate complex at 1.51 A resolution.
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM30A |
| Synchrotron site | ESRF |
| Beamline | BM30A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-11-06 |
| Detector | ADSC CCD |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 77.763, 77.763, 37.896 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 31.203 - 1.507 |
| R-factor | 0.1576 |
| Rwork | 0.156 |
| R-free | 0.17920 |
| Structure solution method | MAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.384 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | SHARP |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.880 | 1.590 |
| High resolution limit [Å] | 1.510 | 1.510 |
| Rmerge | 0.050 | 0.320 |
| Number of reflections | 18899 | |
| <I/σ(I)> | 10.3 | 2.4 |
| Completeness [%] | 99.7 | 98 |
| Redundancy | 13.6 | 12.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.6 | 293 | 0.1 M SODIUM ACETATE PH 4.6, 0.4-1.5 M SODIUM CHLORIDE, 0.00046 M PROTEIN, 0.001.4 M LANTHANIDE COMPLEX, 293 K, 3-7 DAYS |
