4BAD
Hen egg-white lysozyme structure in complex with the europium tris- hydroxymethyltriazoledipicolinate complex at 1.35 A resolution.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-05-27 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 77.403, 77.403, 38.473 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 18.773 - 1.350 |
| R-factor | 0.1527 |
| Rwork | 0.152 |
| R-free | 0.17190 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.815 |
| Data reduction software | XDS (JAUNARY 30 2009) |
| Data scaling software | SCALA (3.3.16) |
| Phasing software | SHARP |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.240 | 1.420 |
| High resolution limit [Å] | 1.350 | 1.350 |
| Rmerge | 0.040 | 0.190 |
| Number of reflections | 26192 | |
| <I/σ(I)> | 12.8 | 3.8 |
| Completeness [%] | 99.7 | 99.2 |
| Redundancy | 7.5 | 6.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.6 | 293 | 0.1 M SODIUM ACETATE PH 4.6, 0.4-1.5 M SODIUM CHLORIDE, 0.00025 M PROTEIN, 0.0027 M LANTHANIDE COMPLEX, 293 K, 3-7 DAYS |
