4BA6
High Resolution structure of the C-terminal family 65 Carbohydrate Binding Module (CBM65B) of endoglucanase Cel5A from Eubacterium cellulosolvens
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I02 |
Synchrotron site | Diamond |
Beamline | I02 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2012-07-16 |
Detector | DECTRIS PILATUS 6M |
Spacegroup name | P 65 |
Unit cell lengths | 83.570, 83.570, 36.750 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 72.370 - 1.420 |
R-factor | 0.17121 |
Rwork | 0.170 |
R-free | 0.19728 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4afm |
RMSD bond length | 0.025 |
RMSD bond angle | 2.495 |
Data reduction software | iMOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 36.750 | 1.500 |
High resolution limit [Å] | 1.420 | 1.420 |
Rmerge | 0.060 | 0.690 |
Number of reflections | 27755 | |
<I/σ(I)> | 11.9 | 1.8 |
Completeness [%] | 99.8 | 99.9 |
Redundancy | 5.3 | 5.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7 | 292 | 2M AMMONIUM SULPHATE, 292 K, pH 7 |