4BA2
Archaeal exosome (Rrp4-Rrp41(D182A)-Rrp42) bound to inorganic phosphate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-01-29 |
Detector | MARRESEARCH |
Spacegroup name | P 21 3 |
Unit cell lengths | 134.830, 134.830, 134.830 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 38.922 - 2.501 |
R-factor | 0.189 |
Rwork | 0.185 |
R-free | 0.25890 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2je6 |
RMSD bond length | 0.008 |
RMSD bond angle | 1.132 |
Data reduction software | XDS |
Data scaling software | XDS |
Refinement software | PHENIX ((PHENIX.REFINE: 1.8_1069)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.650 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.120 | 0.720 |
Number of reflections | 28497 | |
<I/σ(I)> | 14.5 | 2.9 |
Completeness [%] | 1.0 | 1 |
Redundancy | 7.1 | 7.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 8 | 40% PEG 400, 50 MM TRIS-HCL PH 8.0 |