4BA2

Archaeal exosome (Rrp4-Rrp41(D182A)-Rrp42) bound to inorganic phosphate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	CCD
Collection date	2007-01-29
Detector	MARRESEARCH
Spacegroup name	P 21 3
Unit cell lengths	134.830, 134.830, 134.830
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.922 - 2.501
R-factor	0.189
Rwork	0.185
R-free	0.25890
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2je6
RMSD bond length	0.008
RMSD bond angle	1.132
Data reduction software	XDS
Data scaling software	XDS
Refinement software	PHENIX ((PHENIX.REFINE: 1.8_1069))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.650
High resolution limit [Å]	2.500	2.500
Rmerge	0.120	0.720
Number of reflections	28497
<I/σ(I)>	14.5	2.9
Completeness [%]	1.0	1
Redundancy	7.1	7.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		8		40% PEG 400, 50 MM TRIS-HCL PH 8.0