4B9W
Structure of extended Tudor domain TD3 from mouse TDRD1 in complex with MILI peptide containing dimethylarginine 45.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-02-18 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 1 |
| Unit cell lengths | 39.940, 53.960, 60.790 |
| Unit cell angles | 93.69, 98.56, 111.68 |
Refinement procedure
| Resolution | 36.940 - 2.100 |
| R-factor | 0.22262 |
| Rwork | 0.219 |
| R-free | 0.28366 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.377 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.6.0116) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.200 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.110 | 0.390 |
| Number of reflections | 25755 | |
| <I/σ(I)> | 7.28 | 2.55 |
| Completeness [%] | 95.5 | 95.8 |
| Redundancy | 2.4 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | PROTEIN:PEPTIDE 1:5 RATIO, 0.2 M AMMONIUM ACETATE, 0.05 M SODIUM CACODYLATE PH 6.5, 30 % PEG8000, 0.01 M MAGNESIUM ACETATE TETRAHYDRATE |
