4B9P
Biomass sensoring module from putative Rsgi2 protein of Clostridium thermocellum resemble family 3 carbohydrate-binding module of cellulosome
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2011-06-25 |
Detector | DECTRIS PILATUS 6M |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 31.736, 62.289, 79.274 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.978 - 1.182 |
R-factor | 0.1254 |
Rwork | 0.124 |
R-free | 0.14990 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4b97 |
RMSD bond length | 0.013 |
RMSD bond angle | 1.517 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.200 |
High resolution limit [Å] | 1.180 | 1.180 |
Rmerge | 0.090 | |
Number of reflections | 52558 | |
<I/σ(I)> | 16 | 1.6 |
Completeness [%] | 99.9 | 99.8 |
Redundancy | 5.7 | 5.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6.5 | 0.005 M ZNSO4, 0.1 M MES PH 6.5, 23% (V/V) PEG MME 550 |