4B9L

Structure of the high fidelity DNA polymerase I with the oxidative formamidopyrimidine-dA DNA lesion in the pre-insertion site.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2011-05-26
Detector	DECTRIS PILATUS 6M
Spacegroup name	P 21 21 21
Unit cell lengths	87.528, 93.265, 106.107
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.156 - 2.050
R-factor	0.1905
Rwork	0.189
R-free	0.22560
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1u45
RMSD bond length	0.009
RMSD bond angle	1.212
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.370	2.160
High resolution limit [Å]	2.050	2.050
Rmerge	0.150	0.600
Number of reflections	55071
<I/σ(I)>	6.6	2.4
Completeness [%]	99.8	100
Redundancy	4.2	4.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1