4B9J
Structure of self-complemented CssA subunit of enterotoxigenic Escherichia coli colonization factor CS6
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 287 |
| Detector technology | CCD |
| Collection date | 2011-02-24 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 42.998, 48.954, 56.955 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.125 - 2.542 |
| R-factor | 0.2078 |
| Rwork | 0.205 |
| R-free | 0.27710 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4b9i |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.834 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.000 | 2.680 |
| High resolution limit [Å] | 2.540 | 2.540 |
| Rmerge | 0.030 | 0.070 |
| Number of reflections | 4190 | |
| <I/σ(I)> | 33.9 | 16.8 |
| Completeness [%] | 98.8 | 98.3 |
| Redundancy | 3.2 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.6 | 24-30 % PEG4000 IN 0.2 M AMMONIUM ACETATE, 0.1 M NA ACETATE, PH 4.6 |
