4B9A
Structure of a putative epoxide hydrolase from Pseudomonas aeruginosa.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-06-17 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 83.910, 83.910, 140.730 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 27.450 - 1.450 |
| R-factor | 0.11199 |
| Rwork | 0.111 |
| R-free | 0.13771 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1y37 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.758 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 72.080 | 1.530 |
| High resolution limit [Å] | 1.450 | 1.450 |
| Rmerge | 0.100 | 0.800 |
| Number of reflections | 89489 | |
| <I/σ(I)> | 14 | 2.3 |
| Completeness [%] | 100.0 | 99.8 |
| Redundancy | 7.6 | 6.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 0.1 M LISO4, 1.25 M (NH4)2SO4, 0.1M TRIS HCL, PH 8.5 |
