4B97
Biomass sensing modules from putative Rsgi-like proteins of Clostridium thermocellum resemble family 3 carbohydrate-binding module of cellulosome
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2011-02-23 |
Detector | DECTRIS PILATUS 6M |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 34.479, 56.043, 64.594 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 42.331 - 1.276 |
R-factor | 0.1405 |
Rwork | 0.140 |
R-free | 0.15640 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2wnx |
RMSD bond length | 0.012 |
RMSD bond angle | 1.502 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.300 |
High resolution limit [Å] | 1.280 | 1.280 |
Rmerge | 0.060 | 0.450 |
Number of reflections | 33242 | |
<I/σ(I)> | 30.8 | 2.3 |
Completeness [%] | 99.8 | 96.7 |
Redundancy | 6.83 | 5.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 5.6 | 0.2 M AMMONIUM ACETATE, 0.1 M TRI-SODIUM CITRATE PH 5.6, 30 % (V/V) PEG 400 |