4B93
Complex of Vamp7 cytoplasmic domain with 2nd ankyrin repeat domain of Varp
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-01-01 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 69.570, 122.660, 158.600 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.850 - 2.000 |
| R-factor | 0.21505 |
| Rwork | 0.213 |
| R-free | 0.25050 |
| Structure solution method | MIRAS |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.837 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.7.0018) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 56.000 | 2.050 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.100 | 0.960 |
| Number of reflections | 43692 | |
| <I/σ(I)> | 6.9 | 0.9 |
| Completeness [%] | 99.7 | 99.5 |
| Redundancy | 4 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7 | 277 | 5 TO 7.5 % (V/V) ISOPROPANOL AND 0.1 M IMIDAZOLE PH 6.5-7.8 IN HANGING AND SITTING DROPS AT 4 DEGREES |
