4B91
Crystal structure of truncated human CRMP-5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 210 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 90.090, 90.090, 248.340 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.600 - 1.700 |
| R-factor | 0.17168 |
| Rwork | 0.170 |
| R-free | 0.20181 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2gse |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.923 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 51.100 | 1.790 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.090 | 0.600 |
| Number of reflections | 110493 | |
| <I/σ(I)> | 12.9 | 2.3 |
| Completeness [%] | 97.8 | 87.1 |
| Redundancy | 7 | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5 | 23% (V/V) PEG 400 MMT PH 5.0 |
