4B8S
Crystal Structure of Thermococcus litoralis ADP-dependent Glucokinase (GK)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-12-10 |
| Detector | ADSC CCD |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 106.468, 106.468, 130.093 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 34.850 - 2.580 |
| R-factor | 0.17198 |
| Rwork | 0.169 |
| R-free | 0.22056 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1gc5 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.350 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.000 | 2.650 |
| High resolution limit [Å] | 2.580 | 2.580 |
| Rmerge | 0.090 | 0.670 |
| Number of reflections | 33911 | |
| <I/σ(I)> | 18.3 | 3.5 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 7.4 | 7.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.2 | 1.5 M SODIUM CITRATE PH 5.2, 5 MM DTT, 6 MM MG-ADPBETAS AND 30 MM D-GLC. |
