4B8C
nuclease module of the yeast Ccr4-Not complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-10-07 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 1 |
| Unit cell lengths | 122.650, 122.911, 126.421 |
| Unit cell angles | 89.47, 89.74, 64.22 |
Refinement procedure
| Resolution | 47.951 - 3.410 |
| R-factor | 0.2351 |
| Rwork | 0.233 |
| R-free | 0.26970 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4b8a |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.468 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE: 1.7.1_743)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.220 | 3.590 |
| High resolution limit [Å] | 3.400 | 3.400 |
| Rmerge | 0.010 | 0.510 |
| Number of reflections | 90023 | |
| <I/σ(I)> | 11.2 | 2.9 |
| Completeness [%] | 98.5 | 98.4 |
| Redundancy | 3.6 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 8% PEG 3350, 75 MM AMMONIUM ACETATE AND 10% GLYCEROL, pH 7.5 |
