4B8B
N-Terminal domain of the yeast Not1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-04-21 |
| Detector | DECTRIS PILATUS 2M |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 55.097, 164.507, 86.141 |
| Unit cell angles | 90.00, 100.05, 90.00 |
Refinement procedure
| Resolution | 46.050 - 2.801 |
| R-factor | 0.2396 |
| Rwork | 0.238 |
| R-free | 0.27570 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.267 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHENIX (AUTOSOL) |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.700 | 2.950 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.050 | 0.530 |
| Number of reflections | 37032 | |
| <I/σ(I)> | 20 | 2.6 |
| Completeness [%] | 99.5 | 97.1 |
| Redundancy | 4.8 | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.2 | 2.8 M NACL AND 50 MM TRIS PH 7.2 |
