4B8A
Structure of yeast NOT1 MIF4G domain co-crystallized with CAF1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-05-19 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 71.850, 76.560, 109.908 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 60.140 - 2.400 |
| R-factor | 0.1923 |
| Rwork | 0.190 |
| R-free | 0.23600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1uoc |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.052 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.700 | 2.450 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.010 | 0.040 |
| Number of reflections | 27615 | |
| <I/σ(I)> | 15.3 | 4.4 |
| Completeness [%] | 99.5 | 96.5 |
| Redundancy | 6 | 5.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 8% (W/V) PEG 8000, 0.2 M NA MALONATE, 50 MM TRIS PH 8.0 AND 0.1% LOW MELTING AGAROSE |
