4B89
MIF4G domain of the yeast Not1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 104 |
| Detector technology | PIXEL |
| Collection date | 2010-04-24 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 53.758, 53.758, 187.154 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.788 - 1.500 |
| R-factor | 0.1858 |
| Rwork | 0.185 |
| R-free | 0.20820 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | C ALPHA TRACE FROM EXPERIMENTAL (SAD) MAP. |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.993 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHENIX (AUTOSOL) |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 93.580 | 1.480 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rmerge | 0.040 | 0.060 |
| Number of reflections | 54005 | |
| <I/σ(I)> | 22.2 | 2.7 |
| Completeness [%] | 99.8 | 99.6 |
| Redundancy | 7 | 6.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 20% (W/V) PEG3350, 0.5 M NAI AND 50 MM TRIS PH 7.5 |
