4B89

MIF4G domain of the yeast Not1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	104
Detector technology	PIXEL
Collection date	2010-04-24
Detector	DECTRIS PILATUS 6M
Spacegroup name	P 43 21 2
Unit cell lengths	53.758, 53.758, 187.154
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.788 - 1.500
R-factor	0.1858
Rwork	0.185
R-free	0.20820
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	C ALPHA TRACE FROM EXPERIMENTAL (SAD) MAP.
RMSD bond length	0.005
RMSD bond angle	0.993
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHENIX (AUTOSOL)
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	93.580	1.480
High resolution limit [Å]	1.400	1.400
Rmerge	0.040	0.060
Number of reflections	54005
<I/σ(I)>	22.2	2.7
Completeness [%]	99.8	99.6
Redundancy	7	6.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		7.5		20% (W/V) PEG3350, 0.5 M NAI AND 50 MM TRIS PH 7.5