4B7Y
Crystal structure of the MSL1-MSL2 complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-10-25 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 100.990, 100.990, 88.650 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 87.460 - 3.250 |
| R-factor | 0.24527 |
| Rwork | 0.244 |
| R-free | 0.25966 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4b86 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.042 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0116) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 3.370 |
| High resolution limit [Å] | 3.250 | 3.250 |
| Rmerge | 0.080 | 0.680 |
| Number of reflections | 8433 | |
| <I/σ(I)> | 12 | 2.1 |
| Completeness [%] | 98.7 | 99.9 |
| Redundancy | 3.6 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6 | 0.1 M MES PH 6.0 AND 1.6 M AMMONIUM SULFATE |
