4B6W

Architecture of Trypanosoma brucei Tubulin-Binding cofactor B

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	CCD
Detector	ADSC CCD
Spacegroup name	P 41 21 2
Unit cell lengths	50.920, 50.920, 77.220
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.010 - 2.350
R-factor	0.16664
Rwork	0.164
R-free	0.20972
Structure solution method	SAD
Starting model (for MR)	NONE
RMSD bond length	0.014
RMSD bond angle	1.533
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	CRANK
Refinement software	REFMAC (5.6.0119)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.600	2.480
High resolution limit [Å]	2.350	2.350
Rmerge	0.120	0.640
Number of reflections	4631
<I/σ(I)>	15.4	3.3
Completeness [%]	100.0	99.8
Redundancy	12.7	9.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1