4B6H

Structure of hDcp1a in complex with proline rich sequence of PNRC2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-2
Synchrotron site	ESRF
Beamline	ID14-2
Temperature [K]	100
Detector technology	CCD
Collection date	2010-03-16
Detector	ADSC QUANTUM 4
Spacegroup name	I 2 2 2
Unit cell lengths	76.091, 97.639, 109.688
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	72.931 - 2.600
R-factor	0.2388
Rwork	0.234
R-free	0.28470
Structure solution method	SAD
Starting model (for MR)	NONE
RMSD bond length	0.008
RMSD bond angle	1.239
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	SnB
Refinement software	PHENIX ((PHENIX.REFINE: 1.6.1_357))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	72.000	2.600
High resolution limit [Å]	2.600	2.600
Rmerge	0.040	0.170
Number of reflections	12614
<I/σ(I)>	19.1	4.9
Completeness [%]	97.6	91
Redundancy	3.4	2.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1				27% PEG3350, 0.15M AMMONIUM ACETATE, 0.1M BIS TRIS PH5.5