4B6H
Structure of hDcp1a in complex with proline rich sequence of PNRC2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-03-16 |
| Detector | ADSC QUANTUM 4 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 76.091, 97.639, 109.688 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 72.931 - 2.600 |
| R-factor | 0.2388 |
| Rwork | 0.234 |
| R-free | 0.28470 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.239 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SnB |
| Refinement software | PHENIX ((PHENIX.REFINE: 1.6.1_357)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 72.000 | 2.600 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.040 | 0.170 |
| Number of reflections | 12614 | |
| <I/σ(I)> | 19.1 | 4.9 |
| Completeness [%] | 97.6 | 91 |
| Redundancy | 3.4 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 27% PEG3350, 0.15M AMMONIUM ACETATE, 0.1M BIS TRIS PH5.5 |
