4B6D
Structure of the atypical C1 domain of MgcRacGAP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-07-31 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 36.850, 133.850, 56.682 |
| Unit cell angles | 90.00, 109.03, 90.00 |
Refinement procedure
| Resolution | 66.925 - 2.200 |
| R-factor | 0.2125 |
| Rwork | 0.210 |
| R-free | 0.23550 |
| Structure solution method | SAD |
| Starting model (for MR) | 1ptq |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.128 |
| Data scaling software | SCALA |
| Phasing software | SHELXD |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 66.930 | 2.320 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.150 | 0.960 |
| Number of reflections | 26140 | |
| <I/σ(I)> | 9.4 | 2.6 |
| Completeness [%] | 99.4 | 98.8 |
| Redundancy | 9.7 | 8.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.1 | 1.3 M SODIUM CITRATE, PH 7.1 AND 0.3 M DIMETHYLETHYLAMMONIUM PROPANE SULFONATE |
