4B69
A. fumigatus ornithine hydroxylase (SidA) bound to ornithine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | LNLS |
| Synchrotron site | LNLS |
| Temperature [K] | 100 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 77.237, 84.471, 145.276 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.240 - 2.300 |
| R-factor | 0.22001 |
| Rwork | 0.217 |
| R-free | 0.26820 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4b63 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.900 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0119) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.400 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.110 | 0.700 |
| Number of reflections | 21523 | |
| <I/σ(I)> | 24.1 | 3.5 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 14.4 | 13.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.6 | HANGING DROP VAPOR DIFFUSION METHOD, WITH A RESERVOIR SOLUTION CONTAINING; 1.6 M AMMONIUM SULFATE, 0.1 M HEPES, 2% DIOXANE, PH 6.6 |
