4B65
A. fumigatus ornithine hydroxylase (SidA), reduced state bound to NADP(H)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14 |
Synchrotron site | ESRF |
Beamline | ID14 |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | ADSC CCD |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 77.990, 84.460, 145.620 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 72.810 - 2.320 |
R-factor | 0.19877 |
Rwork | 0.196 |
R-free | 0.25013 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4b63 |
RMSD bond length | 0.007 |
RMSD bond angle | 1.109 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.6.0119) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.400 |
High resolution limit [Å] | 2.320 | 2.320 |
Rmerge | 0.080 | 0.210 |
Number of reflections | 21091 | |
<I/σ(I)> | 11.8 | 5 |
Completeness [%] | 99.5 | 98.2 |
Redundancy | 4.1 | 2.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.6 | HANGING DROP VAPOR DIFFUSION METHOD, WITH A RESERVOIR SOLUTION CONTAINING; 1.6 M AMMONIUM SULFATE, 0.1 M HEPES, 2% DIOXANE, PH 6.6. |