4B62
The structure of the cell wall anchor of the T6SS from Pseudomonas aeruginosa
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2011-11-01 |
| Detector | RIGAKU RAXIS II |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 30.240, 51.700, 79.640 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 21.840 - 1.450 |
| R-factor | 0.14126 |
| Rwork | 0.139 |
| R-free | 0.17435 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3khn |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.473 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 21.830 | 1.520 |
| High resolution limit [Å] | 1.450 | 1.450 |
| Rmerge | 0.040 | 0.240 |
| Number of reflections | 22823 | |
| <I/σ(I)> | 14.3 | 3.4 |
| Completeness [%] | 98.8 | 95.1 |
| Redundancy | 3.5 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.9 | SODIUM/POTASSIUM PHOSPHATE PH 6.9 |
