4B61
In meso structure of alginate transporter, AlgE, from Pseudomoas aeruginosa, PAO1. Crystal form 3.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 293 |
Detector technology | CCD |
Collection date | 2009-06-17 |
Detector | MARRESEARCH |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 61.672, 77.463, 240.290 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.865 - 2.402 |
R-factor | 0.2217 |
Rwork | 0.221 |
R-free | 0.24360 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3rbh |
RMSD bond length | 0.019 |
RMSD bond angle | 1.109 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 55.640 | 2.530 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.090 | 0.550 |
Number of reflections | 45680 | |
<I/σ(I)> | 6.9 | 1.8 |
Completeness [%] | 98.6 | 99.9 |
Redundancy | 9.3 | 9.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | LIPIDIC CUBIC PHASE | 6.5 | 0.1 M SODIUM CACODYLATE, PH 6.5, 1.1 M SODIUM ACETATE IN SOLUTION; PROTEIN RECONSTITUTED IN LIPIDIC CUBIC PHASE USING 7.8 MAG AS THE HOSTING LIPID. |