4B5O
Crystal structure of human alpha tubulin acetyltransferase catalytic domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-02-10 |
| Detector | DECTRIS PILATUS |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 42.198, 121.958, 37.277 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.649 - 1.050 |
| R-factor | 0.132 |
| Rwork | 0.131 |
| R-free | 0.15740 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.021 |
| RMSD bond angle | 2.086 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.060 |
| High resolution limit [Å] | 1.050 | 1.050 |
| Rmerge | 0.030 | 0.790 |
| Number of reflections | 86586 | |
| <I/σ(I)> | 29 | 2.3 |
| Completeness [%] | 1.0 | 1 |
| Redundancy | 7.6 | 6.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 50 MM TRIS PH 8.0, 200 MM NAC, 15% PEG8000 |
