4B5J

Neisseria AP endonuclease bound to the substrate with an orphan Adenine base

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Spacegroup name	P 43 21 2
Unit cell lengths	69.880, 69.880, 122.270
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	27.826 - 2.100
R-factor	0.2129
Rwork	0.208
R-free	0.25850
Structure solution method	FOURIER SYNTHESIS
RMSD bond length	0.006
RMSD bond angle	0.761
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall
Low resolution limit [Å]	38.400
High resolution limit [Å]	2.100
Rmerge	0.060
Number of reflections	18365
Completeness [%]	99.1
Redundancy	6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1