4B5H

Substate bound inactive mutant of Neisseria AP endonuclease in presence of metal ions

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	CCD
Detector	ADSC CCD
Spacegroup name	P 43 21 2
Unit cell lengths	68.907, 68.907, 120.968
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	31.065 - 3.050
R-factor	0.1962
Rwork	0.185
R-free	0.29490
Structure solution method	OTHER
Starting model (for MR)	NONE
RMSD bond length	0.003
RMSD bond angle	0.528
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall
Low resolution limit [Å]	59.800
High resolution limit [Å]	3.000
Rmerge	0.100
Number of reflections	5920
Completeness [%]	99.2
Redundancy	5.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1