4B4U
Crystal structure of Acinetobacter baumannii N5, N10- methylenetetrahydrofolate dehydrogenase-cyclohydrolase (FolD) complexed with NADP cofactor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-05-25 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 55.036, 80.126, 68.620 |
| Unit cell angles | 90.00, 107.11, 90.00 |
Refinement procedure
| Resolution | 52.600 - 1.450 |
| R-factor | 0.12319 |
| Rwork | 0.122 |
| R-free | 0.15151 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1b0a |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.478 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 52.600 | 1.530 |
| High resolution limit [Å] | 1.450 | 1.450 |
| Rmerge | 0.060 | 0.420 |
| Number of reflections | 100394 | |
| <I/σ(I)> | 13.1 | 2.9 |
| Completeness [%] | 99.7 | 99.3 |
| Redundancy | 3.6 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.1 M BIS-TRIS PH 5.5, 25 % PEG 3350, 0.2M MGCL AND 2 % DIOXANE |
