4B4N

CPSF6 defines a conserved capsid interface that modulates HIV-1 replication

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Temperature [K]	100
Detector technology	IMAGE PLATE
Detector	MAR scanner 345 mm plate
Spacegroup name	P 21 21 21
Unit cell lengths	40.560, 46.360, 80.820
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.251 - 1.813
R-factor	0.191
Rwork	0.187
R-free	0.27290
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2gon
RMSD bond length	0.015
RMSD bond angle	1.656
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.7.0029)

Data quality characteristics

	Overall
Low resolution limit [Å]	20.000
High resolution limit [Å]	1.800
Number of reflections	13664
<I/σ(I)>	14.5
Completeness [%]	95.8
Redundancy	3.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		290	17 DEGREES (SITTING DROPS).PROTEIN/ PEPTIDE SOLUTION (0.37 MM HIV-1 CAN AND 4 MM CPSF6313-327 IN 20 MM HEPES PH 7, 50 MM NACL, 1 MM DTT) WAS MIXED WITH RESERVOIR SOLUTION (20% W/V PEG 3350, 0.2 M POTASSIUM PHOSPHATE DIBASIC) IN A 1:1 MIX.