4B4D
Crystal structure of FAD-containing ferredoxin-NADP reductase from Xanthomonas axonopodis pv. citri
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM16 |
| Synchrotron site | ESRF |
| Beamline | BM16 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-09-02 |
| Detector | MARRESEARCH |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 50.699, 99.775, 118.785 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 76.400 - 1.500 |
| R-factor | 0.20893 |
| Rwork | 0.207 |
| R-free | 0.22956 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2bgj |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.310 |
| Data reduction software | XDS |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 24.060 | 1.580 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.060 | 0.610 |
| Number of reflections | 46901 | |
| <I/σ(I)> | 13.7 | 1.9 |
| Completeness [%] | 96.7 | 79.8 |
| Redundancy | 4.6 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 4.3 M NACL AND 100 MM HEPES PH 7.5 |
