4B4D
Crystal structure of FAD-containing ferredoxin-NADP reductase from Xanthomonas axonopodis pv. citri
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM16 |
Synchrotron site | ESRF |
Beamline | BM16 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-09-02 |
Detector | MARRESEARCH |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 50.699, 99.775, 118.785 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 76.400 - 1.500 |
R-factor | 0.20893 |
Rwork | 0.207 |
R-free | 0.22956 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2bgj |
RMSD bond length | 0.007 |
RMSD bond angle | 1.310 |
Data reduction software | XDS |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 24.060 | 1.580 |
High resolution limit [Å] | 1.500 | 1.500 |
Rmerge | 0.060 | 0.610 |
Number of reflections | 46901 | |
<I/σ(I)> | 13.7 | 1.9 |
Completeness [%] | 96.7 | 79.8 |
Redundancy | 4.6 | 2.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7.5 | 4.3 M NACL AND 100 MM HEPES PH 7.5 |