4B4A
Structure of the TatC core of the twin arginine protein translocation system
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04-1 |
Synchrotron site | Diamond |
Beamline | I04-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2012-05-30 |
Detector | DECTRIS PILATUS 6M |
Spacegroup name | H 3 2 |
Unit cell lengths | 123.520, 123.520, 216.410 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 22.000 - 3.500 |
R-factor | 0.2535 |
Rwork | 0.252 |
R-free | 0.28820 |
Structure solution method | SAD |
Starting model (for MR) | NONE |
RMSD bond length | 0.010 |
RMSD bond angle | 1.270 |
Data reduction software | XDS (THROUGH XIA2) |
Data scaling software | Aimless (THROUGH XIA2) |
Phasing software | PHENIX.HYSS |
Refinement software | BUSTER (2.11.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 15.000 | 3.600 |
High resolution limit [Å] | 3.500 | 3.500 |
Rmerge | 0.030 | 0.950 |
Number of reflections | 7820 | |
<I/σ(I)> | 13.9 | 1.4 |
Completeness [%] | 96.8 | 96.6 |
Redundancy | 3.3 | 3.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 4 | 0.2M AMMONIUM SULPHATE, 0.02M NACL, 0.02M SODIUM ACETATE PH4.0, 33% V/V PEG 200 |